중간엽줄기세포 부착 및 증식을 위한 poly-l-lysine 도입 PLGA 나노섬유 제조 및 분석

초록

Cell-substrate interaction is fundamentally essential for phenotypic maintenance and multipotency of stem cells. To regulate the cell-substrate interaction, many researchers have tried to mimic the native cellular environment by introducing physical and chemical factors. Electrospinning technique is a useful tool to produce nanofibers with various diameters, porosities and structures like the native cellular physical environment. In electrospinning technique, however,most synthetic biodegradable polymers such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA) and poly(lactic acid-co-glycolic acid) (PLGA) show low adhesion and proliferation of cells on them. Recently, poly-l-lysine (PLL) has been reported as a common coating material for the improvement of cell adhesion. In this study, we hypothesized that PLL introduction in PLGA nanofiber would enhance the adhesion and proliferation of human adipose-derived stem cells (hASCs). To aim this, we fabricated PLGA/PLL nanofiber by electrospinning and evaluated cellular behavior of hASCs on it. PLGA/PLL nanofiber significantly promoted the attachment and proliferation of hASCs without cytotoxicity. Thus, PLGA/PLL nanofiber would be a useful scaffold to enhance adhesion and proliferation of stem cells and presumably to regulate differentiation of them in tissue engineering field.

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