위암의 예후인자로서 Transforming Growth Factor-β Isoforms ( TGF-β1, TGF-β2, TGF-β3) 의 발현에 관한 면역조직화학적 검색

초록

Immunohistochemical studies using polyclonal antibodies to transforming growth factor beta isoforms(TGF-β1, TGF-β2 & TGF-β3), a multifunctional regulatory proteins which hoave effects on normal and transformed cells, were performed on 66 cases of gastric adenocarcinomas in order to analyze the relationship between expression of these isoforms in gastric cancer cells, adjacent mucosa of the cancer and normal control gastric mucosa. In addition to determine the relationship between expression of TGF-β isoforms and various clinicopathological states, including tumor location, histologic types, regional lymph node metastasis and depth of invasion of tumors were carried out. The positive staining reactivity was detected within the cytoplasm and on the cell membrane. The rate of TGF-β isoforms expression in gastric adenocarcinomas were 47% in TGF-β1, 83% in TGF-β2, and 27% in TGF-β3, respectively. The adjacent gastric mucosa from adenocarcinomas and normal control mucosa were 40 % in TGF-β1, 40% and 55% in TGF-β2, 20% and 33% in TGF-β3, respectively. Among the TGF-β isoforms, TGF-β2 was strongly associated with histologic differentiation and regional lymph node metastasis. No significant association was found between expression of TGF-β isoforms and tumor location, histologic types and T-stages. From these results, we can postulate that the altered expression of TGF-β isoforms, especially TGF-β2, play an important role in histogenesis and gastric cancer progression and regional lymph node metastasis.

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