ScholarWorks@중앙대학교

초록

Food allergies have emerged as a significant global public health concern, often triggering rapid and severe reactions upon exposure to allergenic proteins in food. Therefore, the development of accurate, sensitive, and rapid detection strategies is critical for safeguarding public health and ensuring food safety. In this study, we present a high-performance electrochemical biosensor for the detection of the peanut allergen Ara h1, incorporating hydrophilic chitosan nanowhisker–graphene oxide (CSNW–GO) nanohybrids with a phage display-derived unique affinity peptide receptor. The CSNW–GO interface enhances antifouling properties, electron transfer efficiency, and surface stability, while site-specific EDC/NHS coupling enables oriented peptide immobilization to facilitate efficient target capturing and recognition. The characterization and sensing performance of the CSNW–GO assisted electrochemical sensor was studied cyclic voltammetry (CV), circular dichroism (CD), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), field emission transmission electron microscopy (FE-TEM), surface plasmon resonance (SPR), X-ray photoelectron spectroscopy (XPS) and rheological measurements. The biosensor demonstrates a broad detection range and a low detection limit of 0.42 ng/mL, along with excellent selectivity against non-target proteins. Notably, Ara h1 was accurately quantified in complex food matrices, highlighting the biosensor's potential for on-site allergen monitoring and broader biosensing applications.

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