Dyadobacter pollutisoli sp. nov., isolated from plastic waste landfill soil

초록

A Gram-stain-negative, yellow-pigmented and facultatively aerobic bacterium, designated strain U1T, was isolated from plastic dumped soil sampled in the Republic of Korea. Cell of strain U1T were non-motile rods showing catalase-negative and oxidase-positive activities. Strain U1T was shown to grow at 10–37 °C (optimum, 25–30 °C) and pH 6.0–9.0 (optimum, pH 8.0), and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Strain U1T contained iso-C15:0, C16:0, C16:1 ω5c and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c) as the major cellular fatty acids (>5%) and menaquinone-7 as the sole respiratory quinone. Phos-phatidylethanolamine, two unidentified aminolipids and three unidentified lipids were identified as the major polar lipids. The DNA G+C content of strain U1T calculated from the whole-genome sequence was 45.5mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain U1T formed a distinct phylogenetic lineage within the genus Dyadobacter. Strain U1T has the highest 16S rRNA sequence similarity to Dyadobacter bucti QTA69T (97.9%). Average nucleotide identity and digital DNA–DNA hybridization values between strain U1T and D. bucti QTA69T were 74.6% and 18.9%, respectively. Based on pheno-typic, chemotaxonomic and molecular features, strain U1T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter pollutisoli sp. nov. is proposed. The type strain is U1T (= KACC 22210T=JCM 34491T). © 2023 The Authors.

키워드