THEORY OF DIFFUSION-INFLUENCED FLUORESCENCE QUENCHING - EFFECTS OF STATIC QUENCHING ON THE STERN-VOLMER CURVE

초록

A theory for describing the effects of static quenching in the diffusion-influenced fluorescence quenching is presented, which is based on a hierarchical system of many-body Smoluchowski equations for the reactant molecule distribution functions. The present theory describes the dependence of fluorescence quenching dynamics on the complex formation between the ground-state fluorophor and quencher molecules and on the intensity of external illumination within a unified framework. In the conventional Smoluchowski approach, it has been simply assumed that the intensity of illumination should be quite weak, and the influence of the complex formation has been considered only from a static viewpoint. A model calculation shows that the light intensity effect on Stem-Volmer curves should be more pronounced in the presence of static quenching.

키워드