Preparation of biodegradable PLGA nanospheres employing a fast solvent evaporation method

초록

The traditional W-1/O/W-2 emulsion method has a limit on the control of particle size to a nano-scale with high drug encapsulation efficiency (EE%). In the present study, we modified the solvent removal time and rate to develop an oral peptide drug delivery system using sCT as a model drug. We monitored the effect of the type of poly (vinyl alcohol) (PVA) within the W-2 phase on the EE%. SEM images and particle size measurements revealed that the outer surfaces of representative nanosphere samples were very fine and even; the particle size was 383.2 +/- 8.7 nm with a narrow size distribution (polydispersity index = 0.25 +/- 10.004). The EE% increased depending on the solvent removal time and rate from 11.4 +/- 1.3 to 28.8 +/- 3.0 and 52.5 +/- 6.5, compared to control nanospheres, respectively. The EE% of sCT was also enhanced by controlling the molecular weight of PVA within the W-2 phase.

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