LaSrO3 perovskite quantum dots as a fluorescent probe for the detection of bilirubin and epinephrine via FRET and IFE mechanisms

초록

In this study, blue-emitting LaSrO3 perovskite quantum dots (PQDs) were synthesized using a microwave-assisted sol–gel method. The as-prepared LaSrO3 PQDs acted as a nano-fluorescent probe for detection of two biomarkers: bilirubin (BR) and epinephrine (EP). The fluorescence spectra of LaSrO3 PQDs showed λEm/Ex at 421/336 nm, displaying quantum yield of 36.91 %. Based on the remarkable fluorescence properties of LaSrO3 PQDs, fluorescence sensing studies were performed for biomarkers assay. The emission spectral results of LaSrO3 PQDs displayed remarkable fluorescence quenching by BR and EP via Förster resonance energy transfer and inner filter effect, respectively. The detection limits were 10.48 and 17.04 nM for BR and EP, respectively. The fluorescence quenching mechanisms were discussed in detail by various analytical techniques (fluorescence, UV–visible absorption, transmission electron microscopy, Fourier transform infrared, and zeta potential). In addition, LaSrO3 PQDs-based fluorescence sensing approach was successfully applied to quantitative assays of EP in pharmaceutical samples and of BR in biofluids with satisfactory recovery results. © 2024 Elsevier B.V.

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