Solitalea longa sp nov., isolated from freshwater and emended description of the genus Solitalea

초록

A Gram-negative, strictly aerobic, oxidase-positive, catalase-negative and yellow-pigmented bacterium, designated strain HR-AV(T), was isolated from a water sample of the Han River. Cells were elongated rods with gliding motility without flagellum. Growth was observed at 5-30 degrees C (optimum, 20 degrees C), pH 7-8 and 0-0.5% NaCl. The major respiratory quinone was menaquinone-7. The major fatty acids were iso-C-15 : 0, summed feature 3 (comprising C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and anteiso-C-15 (: 0). The polar lipids comprised phosphatidylethanolamine, an unidentified amino lipid and five unidentified lipids. The DNA G+C content of strain HR-AV(T) was 38.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AV(T) belonged to the family Sphingobacteriaceae in the phylum Bacteroidetes and formed a phylogenic lineage with Solitalea canadensis DSM 3403(T) and Solitalea koreensis R2A36-4(T). Strain HR-AV(T) was most closely related to S. canadensis DSM 3403(T) and S. koreensis R2A36-4(T) with 97.3 and 94.0% 16S rRNA gene sequence similarities, respectively, and then had low similarities (below 90.9 %) with other bacteria with validly published names. Average nucleotide identity and in silico DNA-DNA hybridization values between strain HR-AV(T) and S. canadensis were 74.0 and 19.7 %, respectively. Based on these results, strain HR-AV(T) represents a novel species of the genus Solitalea, for which the name Solitalea longa sp.nov. is proposed. The type strain is HR-AV(T) (=KACC 19411(T)=JCM 32259(T)). An emended description of the genus Solitalea is also proposed.

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