Staphylococcus parequorum sp. nov. and Staphylococcus halotolerans sp. nov., isolated from traditional Korean soybean foods

초록

Strains Mo2-6T, S9, KG4-3T, and 50Mo3-2, identified as coagulase-negative, Gram-stain-positive,halotolerant, non-motile coccoid bacteria, were isolated from traditional Korean soybeanfoods. Strains Mo2-6T and S9 were both catalase- and oxidase-negative, whereas KG4-3T and 50Mo3-2 were catalase-positive but oxidase-negative. The optimal growth conditionsfor Mo2-6T and S9 were 30°C, 2% NaCl, and pH 7.0, while KG4-3T and 50Mo3-2 grew best at35°C, 2% NaCl, and pH 7.0. All strains contained menaquinone-7 as the predominant isoprenoidquinone, with anteiso-C15:0 and iso-C15:0 as the major cellular fatty acids (> 10%). Additionally,anteiso-C13:0 was a major fatty acid in strain KG4-3T. The DNA G + C contents ofstrains Mo2-6T, S9, KG4-3T, and 50Mo3-2 were 33.4%, 33.3%, 32.5%, and 32.7%, respectively. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealedthat strains Mo2-6T and S9, as well as KG4-3T and 50Mo3-2, formed distinct lineages withinthe genus Staphylococcus. Digital DNA-DNA hybridization (dDDH) and average nucleotideidentity (ANI) analyses confirmed that strains Mo2-6T and S9, as well as KG4-3T and 50Mo3-2,belonged to the same species. Meanwhile, dDDH and ANI values between strains Mo2-6Tand KG4-3T, as well as comparisons with other Staphylococcus type strains, were below thespecies delineation thresholds, indicating they represent novel species. Based on phenotypic,chemotaxonomic, and molecular data, we propose strain Mo2-6T as the type strain ofStaphylococcus parequorum sp. nov. (=KACC 23685T =JCM 37038T) and strain KG4-3T asthe type strain of Staphylococcus halotolerans sp. nov. (=KACC 23684T =JCM 37037T).

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