Green synthesis of copper nanoclusters using Cissus quadrangularis leaves extract and cysteine as cooperative ligands for the detection of Fe 3+ion and 2,6-dipicolinic acid in biofluids via fluorescence turn off-on mechanism

초록

An off -on fluorescent sensor is devised by using greenish-blue light-emitting Cissus quadrangularis-cysteine- CuNCs ( C. quadrangularis-Cys-CuNCs) for the rapid, precise, and recognition of Fe 3 + ion and 2,6-dipicolinic acid (DPA). The structure, size, surface functionalities and oxidation state of the elements present in C. quadrangularis- Cys-CuNCs are confirmed by high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopic and X-Ray diffraction (XRD) techniques. The as-synthesized C. quadrangularis-Cys-CuNCs exhibited lambda Em/Ex at 491/370 nm. The fluorescence intensity of C. quadrangularis- Cys-CuNCs was reduced selectively to 63 % by Fe 3 + , however it was restored with the addition of DPA. This method exhibited the limits of detection (LODs) of 0.125 and 0.38 mu M for Fe 3 + and DPA, respectively. The C. quadrangularis-Cys-CuNCs based fluorescence turn-off -on sensing platform showed remarkable performance for the analyses of Fe 3 + ion and DPA in real samples, revealing great potentiality of the method for practical applications.

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