RDNA NTS BASED GENE MULTIPLE INSERTION CASSETTE SET AND GRAS-GRADE RECOMBINANT YEAST STRAIN

초록

The present invention relates to a ribosomal DNA nontranscribed spacer (rDNA NTS)-based gene multiple insertion cassette set and a GRAS-grade recombinant yeast strain using the same. In particular, a gene multiple insertion cassette set including rDNA NTS fragments and an auxotrophic selection marker having an incomplete promoter is developed, and a safe oral recombinant strain having no antibiotic resistant marker is constructed by multiple insertion of an optimum number of the developed gene multiple insertion cassette sets into chromosomes of a Saccharomyces cerevisiae strain. In addition, the present invention provides a vaccine composition including, as an active ingredient, the above strain, a culture product thereof, a cell lysate, or nodavirus capsid protein (NNVcp) isolated and purified therefrom. In addition, the present invention provides a composition for feed addition including, as an active ingredient, the above strain, a culture product thereof, a cell lysate, or squalene or oxidosqualene isolated and purified therefrom. Furthermore, the present invention provides a technique of preparing a GRAS-grade recombinant yeast strain for mass production of various recombinant proteins or useful metabolites.