Spatial and temporal distribution of N-type Ca2+ channels in gerbil global cerebral ischemia

초록

In the present study, we have investigated the spatial and temporal distribution of voltage gated calcium channels in the gerbil model of global cerebral ischemia using immunohistochemistry. Distinct localizations of P-type (alpha (1A)), N-type (alpha (1B)), and L-type (alpha (1C) and alpha (1D)) Ca2+ channels were observed in the hippocampus at days 1-5 after ischemic injury. However, increased expression of N-type Ca channels was detectable in brain regions vulnerable to ischemia only at days 2 and 3 after ischemic injury. The pyramidal cell bodies of CA1-3 areas and the granule cell bodies of the dentate gyrus were intensely stained at days 2 and 3 following ischemic injury. Transient changes in N-type Ca2+ channel expression were also observed in the affected cerebral cortex and striatum at days 2 and 3 after ischemic injury. Although the present study has not addressed the multiple mechanisms contributing to the intracellular free Ca2+ concentration ([Ca2+](i)) increase in the ischemic brain, the first demonstration of the transient increase in N-type Ca2+ channels may prove useful for future investigations. (C) 2001 Published by Elsevier Science B.V.

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