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GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
- Ubiyvovk, Vira M.;
- Nazarko, Taras Y.;
- Stasyk, Olena G.;
- Sohn, Min Jeong;
- Kang, Hyun Ah;
- 외 1명
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15초록
The GSH2 gene, encoding Hansenula polymorpha, gamma-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
키워드
- 제목
- GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha
- 저자
- Ubiyvovk, Vira M.; Nazarko, Taras Y.; Stasyk, Olena G.; Sohn, Min Jeong; Kang, Hyun Ah; Sibirny, Andrei A.
- 발행일
- 2002-08
- 유형
- Article; Proceedings Paper
- 권
- 2
- 호
- 3
- 페이지
- 327 ~ 332