Preparation of redox-sensitive β-CD-based nanoparticles with controlled release of curcumin for improved therapeutic effect on liver cancer in vitro

초록

A redox-sensitive beta-cyclodextrin nanoparticle (b-CD NP) system was prepared for the controlledrelease of curcumin (CUR), and its therapeutic efficacy on liver cancer was compared with that of CUR invitro. 1-Dodecanethiol (DDT) was conjugated to per-6-thiol-b-CD by disulfide bond formation though anoxidation reaction (b-CD-ss-DDT). Rhodamine B (RhoB) was included into the b-CD-ss-DDT cavity byinclusion complex formation (b-CD-ss-DDT-ic-RhoB). CUR was encapsulated into b-CD-ss-DDT-ic-RhoBaggregates by self-assembly through dialysis method. The composition, morphology, size distributionand zeta potential of b-CD-ss-DDT-ic-RhoB NPs were characterized by proton nuclear magneticresonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy(TEM) and dynamic light scattering (DLS), respectively. It was found that CUR was released from b-CDss-DDT-ic-RhoB/CUR NPs due to the dissociation of b-CD-ss-DDT through a reduction reaction. b-CD-ss-DDT-ic-RhoB/CUR NPs had a higher cellular uptake ratio and a greater anticancer effect on mousehepatoma Hepa 1-6 cells than CUR, mainly attributed to the improved water-solubility of CUR afterencapsulating it in the NPs. Our findings suggest that b-CD-ss-DDT-ic-RhoB NPs can be used asnanocarriers for delivering CUR into cancer cells, thereby serving as a clinical therapeutic agent for livercancer treatment.

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