ScholarWorks@중앙대학교

초록

Multiple biomarkers associated with atopic dermatitis were simultaneously quantified using Ag nanorods on micro post arrays (Ag NMPA). The Ag NMPA substrate consists of 500 nm-high metallic nanorods formed on top of 50 μm-high microstructures. This was fabricated utilizing UV micro-imprinting and glancing angle deposition (GLAD). Ag NMPA enhances fluorescence signal intensity through the metal-enhanced fluorescence (MEF) effect of its metallic nanostructures. Additionally, the microscale height differences improve the signal-to-background noise ratio and help define the detection area with high specificity. The performance of the fabricated Ag NMPA was comparatively evaluated using commercial ELISA kits and epoxy glass microarrays. The results showed that the Ag NMPA exhibited linearity comparable to that of the ELISA kit over the same concentration range (R2 > 0.99), while providing a significantly improved linear fit and up to a 148-fold increase in fluorescence signal compared with the epoxy glass microarray using the same detection strategy. In an accuracy test using custom mixtures of standard samples at arbitrary concentrations, the Ag NMPA demonstrated quantitative accuracy exceeding 88%. Finally, a quantitative test of 24 atopic dermatitis patient samples and 13 healthy control samples revealed comparable results between the Ag NMPA and the ELISA kit. This confirms the potential of the developed Ag NMPA as a practical platform for the simultaneous quantitative analysis of multiple biomarkers in clinical applications.

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