Pacificibacter aestuarii sp nov., isolated from a tidal flat

초록

A Gram-stain-negative, strictly aerobic bacterium, designated KJ21(T), was isolated from a tidal flat in South Korea. Cells were non-motile rods showing oxidase-and catalase-positive activities. Growth of strain KJ21(T) was observed at 10-35 degrees C (optimum, 30 degrees C), at pH 6.0-8.5 (optimum, pH 7.0) and in the presence of 1-5% (w/v) NaCl (optimum, 2 %). Strain KJ21(T) contained summed feature 8 (comprising C-18 : 1 omega 7c/C-18 : 1 omega 6c), C-16 : 0, 10-methyl C-19 : 0 and C-10 : 0 3-OH as the major fatty acids and ubiquinone-10 as the major isoprenoid quinone. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown aminolipid and an unknown lipid were detected as the major polar lipids. The G+C content of the genomic DNA was 53.9 mol%. Phylogenic analysis based on 16S rRNA gene sequences showed that strain KJ21(T) formed a tight phylogenetic lineage with the members of the genus Pacificibacter with a 100% bootstrap value. Strain KJ21(T) was most closely related to Pacificibacter maritimus KMM 9031(T) (98.7 %) and Pacificibacter marinus HDW-9(T) (98.4 %), and the DNA-DNA relatedness values between strain KJ21(T) and the type strains of P. maritimus and P. marinus were 46.9 +/- 4.2% and 39.8 +/- 5.7, respectively. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain KJ21(T) represents a novel species of the genus Pacificibacter, for which the name Pacificibacter aestuarii sp. nov. is proposed. The type strain is KJ21(T) (= KACC 19098(T) = JCM 31805(T)).

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