쥐 태자 간에서 혈구형성세포의 활성도와 분화에 관한 광학 및 전자현미경적 연구

초록

This study was designed to investigate the fetal rat hepatic hemopoiesis with age. Time appearance, patterns of hemopoietic activities and ultrastructural changes of each hemopoietic cell were observed. Erythropoietic activity began to appear by the 13 day of gestation, reached the highest by the 17 day of gestation and thereafter declined. The nucleus of proerythroblast had slight peripheral chromatic along the inner nuclear membrane and one or two nucleoli. The cytoplasm had Golgi apparati, RERs, mitochondria and numerous free ribosomes. In accordance with the development, the size of erythropoietic cell became smaller as well as the one of nucleus. Progressive clumping chromatin along the nuclear membrane with decrease in intracellular organelles were seen, and finally the enucleation began. Not frequently the erythropoietic cells showed bristle coated vesicles in the plasmalemma. Eosinophilic granulocytopoietic cells were found for the first time in hepatic laminae by the 15 day of gestation and the activity gradually increased with age. On the view point of differentiation, promyelocyte had spherical nucleus with granular chromatin, abundant RERs, free ribosomes, mitochondria and nonspecific granules. More differentiated granulocytopoietic cells were also found with specific and non-specific granules.Megakaryoblast appeared first by the 13 day of gestation and the activity abruptly reached the highest level at 14 day of gestation and then decreased. Megakaryocytes were composed of dense core vesicles, dilatated RERs, oval mitochondria, abundant ribosomes and 3 to 5 nuclei. Especially megakaryocytes containing erythrocytes and granulocytes in their cytoplasms were frequently observed. With these morphological basis, the origin of hepatic hemopoietic cell, erythropoietin and other substances influencing the hemopoiesis, and stromal cells which are the components of hemopoietic microenvironment were discussed.

키워드