호도약침액이 가토 뇌조직의 Na+-pump 활성 장애 개선에 미치는 영향

초록

This study was undertaken to determine whether Juglandis Semen (JAS) extract exerts protective effect against oxidant-induced inhibition of Na+-pump activity in cerebral cortex. Na+-pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extract. The oxygen consumption was increased by an increase in Na+concentration from 5 to 100 mM, K+concentration from 0.5 to 10 mM, and Mg2+concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effect of tBHP and protective action of JAS on oxygen consumption. tBHP (1 mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These results suggest that JAS exerts protective effect against tBHP-induced inhibition of Na+-pump activity in the cerebral cortex, probably through action as antioxidant.

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