In vitro anti-inflammatory activity of Russula virescens in the macrophage like cell line raw 264.7 activated by lipopolysaccharide

초록

Four different concentrations of Russula virescens (RVE) (0.5, 1, 2.5 and 5 mg/ml) were extracted by using ethanol. The results showed that the RVE concentrations of 5 mg/ml in the presence of lipopolysaccharide (LPS) (2 μg/ml) affected cell viability. The amount of nitrite, a stable metabolite of nitric oxide (NO) was used as the indicator of NO production in the medium. During the 24 h incubation, nitrite was 0.9 μM in control. When LPS (2 μg/ml) was added, NO production was 2.7 μM whereas, LPS-induced NO production was reduced in 1~5 mg/ml RVE treated groups. The tumor necrosis factor- α (TNF-α) mRNA expression in the RAW 264.7 cell decreased in the 5 mg RVE treated sample. Expressions of Signal transducers and activators of transcription (STAT) 1, pSTAT1, STAT6 and pSTAT6 were decreased by the RVE treatment in a dose-dependent manner in the RAW 264.7 cell. All these results suggest that the ethanol-extract of the RVE mushroom is a potent immune modulator in the RAW 264.7 cell.

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