Research on the Efficient Preparation Method of Metal-enhanced Fluorescent Composite Structure Microarray Chip

초록

Microarray chip with micro-nano composite structure consists of an array of micropost structures and nanorods covering the top of the posts. This structure can significantly enhance the signal-to-noise ratio and improve the cross-infection problem to increase the detection sensitivity and accuracy. However, the construction of such structures is still limited to the laboratory research stage, which is mainly realized by UV nanoimprinting and glancing angle deposition methods, and the preparation process is complicated, time-consuming, and inefficient, which greatly restricts the wide application of highly sensitive microarray chips. Therefore, to achieve high accuracy and sensitivity in the large-scale, high-efficiency production of microarray chips, a micropost array structure was fabricated via injection molding. The top of the micropost structure (spotting area) was selectively deposited with Ag nanorods using glancing angle deposition, forming a micro-nano composite structure substrate. Despite a 4 μm discrepancy between the height of the micropost structure on the microarray chip (54 μm) and its designed height (58 μm), the deviation was within acceptable limits. While the uniformity of Cy-3 fluorescence signal across the micro-nano composite structure substrate (with an average signal value of 12519.0 and a coefficient of variation of 4.79%) was slightly lower than that of the UV nanoimprinting chip (with an average signal value of 12495.8 and a coefficient of variation of 1.35%), both exhibited similar fluorescence signal enhancement characteristics. Therefore, this method can serve as a reference for the high-throughput, efficient production of microarray chips. © 2024 SPIE.

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