Secretory expression of human alpha(1)-antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer

초록

Human alpha(1)-antitrypsin (alpha(1)-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of alpha(1)-AT, though the secretion efficiency was decreased. The final titre of alpha(1)-AT secreted and the secretion efficiency were 17.9 mu g/ml and 24.8%, respectively, with 0.5 g galactose/l.

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