Mini-gel을 이용한 비방사성 SSCP의 위암 p53유전자 변이 검출

초록

Background/Aims: p53 mutation was reported in many studies, but conventional PCR-SSCP protocol using radiolabeled PCR primers or nucleotides to generate a radioactive PCR products is cumbersome to handle. With the aim of a rapid and safe method for single-strand conformation polymorphism(SSCP) analysis of PCR products, we used Mini-SSCP method to detect the mutation of p53 gene of gastric cancer. Methods: In 27 pair of gastric cancer and normal tissues, PCR-SSCP was done with Mini-SSCP, and then Silver sequencing was done. Results: The p53 gene mutation of gastric cancer was found in 10 of 27(37%). Mutation of exon 4, 5 were 2, 5 cases and tnutation of exon 6, 7 mutation were 0, 3 cases respectively. Mutation rate was similar with other reports. P53 mutation was not correlated with stage, lymph node involvement and histological differentiation. Conclusions: Mini-SSCP method, modification of Cold-SSCP, greately increased our accessibility to detect polymorphisms with easiness and safeness. So, it is a useful method to detect genetic mutation. (Korean J Gastroenterol 1997;29:601-609)

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