Flavobacterium agrisp. nov., a novel bacterial species isolated from rhizospheric soil ofCoriandrum sativum

초록

A Gram-stain-negative, strictly aerobic, cream yellow colored, non-motile, rod-shaped bacterial strain, designated MAH-1(T)was isolated from rhizospheric soil ofCoriandrum sativum. A polyphasic taxonomic study was performed on the isolated strain. Optimal growth occurred at 28-30 degrees C, pH 6.5 and 0% NaCl. The strain showed activity for both catalase and oxidase tests. Cell growth occurs on R2A agar, nutrient agar and Luria-Bertani agar. Cells were able to hydrolyze starch, aesculin, gelatin, and Tween 20. Alignment of 16S rRNA gene sequences indicated that strain MAH-1(T)was associated with the genusFlavobacteriumand was most closely related toFlavobacterium longumYIT 12745(T)(94.5% sequence similarity) andFlavobacterium caeniLM5(T)(93.0%). Strain MAH-1(T)had a genome size of 3,975,600 bp. Genome contained 67 contigs encoded by 3,522 protein-coding genes with 38 tRNA and 6 rRNA genes. The genomic DNA G + C contents of strain MAH-1(T)was 47.1 mol %. The genomic ANI and dDDH values between strain MAH-1(T)and one of the close relativesF. caeniLM5(T)were 72.2 and 18.8%, respectively. The major fatty acids were C-15:0 iso, C(16:0 iso)and C-15:0 anteiso. The predominant respiratory quinone was menaquinone 6 (MK-6). Based on physiological, biochemical and phylogenetic data for this isolate, it was confirmed that strain MAH-1(T)was affiliated to the genusFlavobacteriumand represented a novel species, for which the nameFlavobacterium agrisp. nov. is proposed. The type strain is MAH-1(T)(= KACC 19300(T) = CGMCC 1.16617(T)).

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