Tessaracoccus arenae sp nov., isolated from sea sand

초록

A Gram-stain positive, non-spore-forming, non-motile, facultatively anaerobic bacterial strain, designated CAU 1319(T), was isolated from sea sand and the strains taxonomic position was investigated using a polyphasic approach. Strain CAU 1319(T) grew optimally at 30 degrees C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain CAU 1319T belongs to the genus Tessaracoccus , and is closely related to Tessaracoccus lapidicaptus IPBSL-7(T) (similarity 97.69 %), Tessaracoccus bendigoensis Ben 106(T) (similarity 95.64 %) and Tessaracoccus flavescens SST-39T (similarity 95.84 %). Strain CAU 1319T had ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9 (H-4) as the predominant menaquinone, and anteiso-C-15 : 0 as the major fatty acid. The polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, two unidentified aminolipids, three unidentified phospholipids and one unidentified glycolipid. Predominant polyamines were spermine and spermidine. The DNADNA hybridization value between strain CAU 1319(T) and T. lapidicaptus IPBSL-7(T) was 24 %+/- 0.2. The DNA G+C content of the novel strain was 69.5 mol%. On the basis of phenotypic and chemotaxonomic properties, as well as phylogenetic relatedness, strain CAU 1319Tshould be classified as a novel species of the genus Tessaracoccus , for which the name Tessaracoccus arenae sp. nov. is proposed. The type strain is CAU 1319(T)(=KCTC 39760(T)=NBRC 111973(T)).

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