Geomonas soli gen. nov., sp. nov., a New Member of the Family Comamonadaceae, Isolated from Soil

초록

A Gram-stain negative, facultative aerobic bacterial strain, designated strain S-16(T), was isolated from soil in South Korea. Colonies were white-milkish and cells were non-motile rods with oxidase- and catalase-positive activities. The growth of strain S-16(T) was observed at 20-40 degrees C (optimum, 25-30 degrees C) and pH 5.5-7.0 (optimum, pH 6.5). Ubiquinone-8 was identified as the sole respiratory quinone and C-12:0, C-16:0, C-18:0, C-15:1 omega 5c and summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c) were identified as the major fatty acids (>5%). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified polar lipid. The G + C content of the genomic DNA calculated from the whole genome sequence was 66.8 mol%. Strain S-16(T) was most closely related to Piscinibacter aquaticus IMCC1728(T), Rhizobacter gummiphilus NS21(T) and Rhizobacter dauci H6(T) with 16S rRNA gene sequence similarities of 97.93%, 97.93% and 97.44%, respectively. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences suggested that strain S-16(T) could form a distinct phyletic lineage as a new genus within the family Comamonadaceae. Based on the phenotypic, chemotaxonomic and molecular features, strain S-16(T) represents the type strain of a novel species of a novel genus within the family Comamonadaceae, for which the name Geomonas soli gen. nov., sp. nov. is proposed. The type strain is S-16(T) (= KACC 19792(T) = JCM 32971(T)).

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