Development of a liquid-based cytology method for detecting cervical cancer cells using functional gold nanorods

초록

A cytopathology test for cervical cancer diagnosis has been introduced to complement the histopathologytest. This study developed a new cervical cancer diagnostic method that complements the existing liquid cytology toreduce the diagnostician’s skill dependence and to improve diagnostic accuracy. With the ease of introduction of surfacefunctional groups and their high biocompatibility and stability, gold nanomaterials have been used to diagnosenumerous biomolecules. CA125 and p16 markers were chosen as protein markers uniquely present in cervical cancer. Bioconjugation technology was used to form a stable bond between the target antibody and gold nanorods. Antibodygoldnanorods were administered to cervical cancer cells as a factor for optical microscopic analysis. The degree of cancercell labeling was determined through additional fluorescence microscopy analysis. Additionally, a liquid-based cytologytest mimicking the clinical environment was prepared, and the false-positive rate, false-negative rate, and accuracywere determined when the study strategy was implemented. The strategy validated in this study (antibody-gold nanorodbasedcervical cancer diagnosis) suggests a method that can reduce the dependence on experts for the cancer cytologyand can increase test accuracy.

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