Metabolic analysis of guava (Psidium guajava L ) fruits at different ripening stages using different data-processing approaches

초록

Gas chromatography coupled with time-of-flight mass spectrometry and principal component analysis were used to obtain the metabolite profiles of guava (Psidium guajava) fruits Results with two types of data-processing software ChromaTOF and AMDIS were compared to explain the differences between the samples There were some differences in score and loading plot patterns of PCA as well as in the composition of the metabolites However little difference was observed in the type of metabolites detected and identified using either type of software Both the flesh and peel of premature and mature white guava fruits were compared for the analysis of the metabolite profiles Malic acid aspartic acid and glucose were the major metabolites distinguishing the different parts of guava fruits in the PCA loading plot In addition the metabolic profiles of the fruits revealed significant changes in some metabolites during ripening The major components contributing to the separation were serine citric acid fructose sucrose and some unknowns In particular sucrose fructose serine and citric acid were related to the ripening of guava fruits Fructose and sucrose were increased whereas citric acid was decreased during guava fruit ripening (C) 2010 Elsevier B V All rights reserved

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