Enhanced growth, chlorophyll a and phycobiliprotein content, and modulation of bioactive metabolite profiles in Synechocystis sp. PCC 6803 culture by (+)-usnic acid

초록

Synechocystis 6803 is a strain of unicellular freshwater cyanobacteria that can grow in both phototrophic and mixotrophic conditions. We investigated the effects of exogenous ( +)-usnic acid (0.01, 0.1, and 1 mu M) on the growth, photosynthetic pigments, and metabolic profiles of Synechocystis 6803 culture through gas chromatography-mass spectrometry analysis (GC-MS) coupled with multivariate statistical analysis. Treatment with 1 mu M ( +)-usnic acid enhanced the cell growth at day 14 and the photosynthetic pigments and relative levels (intensity g(-1)) of various bioactive metabolites such as glycine, serine, palmitoleic acid, alpha-linolenic acid, linoleic acid, oleic acid, stearic acid, 1-monopalmitin, glycerol monostearate, neophytadiene, and alpha-tocopherol at days 8 and 14. Moreover, enhanced productivity of alpha-tocopherol was achieved on days 8 (0.36 +/- 0.03 mg L-1 or 348 +/- 28 mu g g(-1)) and 14 (0.47 +/- 0.05 mg L-1 or 457 +/- 52 mu g g(-1)) when treated with 1 mu M usnic acid. These results suggest that Synechocystis 6803 can be treated with ( +)-usnic acid to enhance applications in foods, cosmetics, and pharmaceutical industries.

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