ScholarWorks@중앙대학교

초록

A Gram-stain-negative, aerobic, non-motile, rod-shaped, and orange-pigmented bacterium,designated CJ426T, was isolated from ginseng soil in Anseong, Korea. Strain CJ426T grew optimallyon Reasoner’s 2A agar at 30°C and pH 7.0 in the absence of NaCl. Phylogenetic analysisbased on the 16S rRNA gene sequence revealed that strain CJ426T belonged to the familyChitinophagaceae and had the highest sequence similarity with Niabella hibiscisoli KACC18857T (98.7%). The 16S rRNA gene sequence similarities with other members of the genusNiabella ranged from 92.3% to 98.1%. Phylogenomic analyses and overall genomic relatednessindices, including average nucleotide identity, average amino acid identity, and thepercentage of conserved proteins values, supported the classification of strain CJ426T as arepresentative of a novel genus within the family Chitinophagaceae. Furthermore, genome-based analyses suggested that five members of the genus Niabella, including N. aquatica, N. defluvii, N. ginsengisoli, N. hibiscisoli, and, N. yanshanensis, should be separatedfrom other Niabella species and be assigned as a novel genus. The major isoprenoidquinone of strain CJ426T was menaquinone-7 (MK-7). The predominant polar lipids werephosphatidylethanolamine and six unidentified aminolipids. The major fatty acids wereiso-C15:0, iso-C15:1 G, and iso-C17:0 3-OH. The genome of strain CJ426T was 6.3 Mbp in size, consistingof three contigs, with a G + C content of 41.9%. Based on a polyphasic taxonomic approach,strain CJ426T represents a novel genus and species within the family Chitinophagaceae,for which the name Paraniabella aurantiaca gen. nov., sp. nov. is proposed. The typestrain is CJ426T (= KACC 23908T = JCM 37728T).

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