Inactivation of murine norovirus-1 in the edible seaweeds Capsosiphon fulvescens and Hizikia fusiforme using gamma radiation

초록

This study investigated the effects of gamma radiation (3-10 kGy) upon the inactivation of murine norovirus-1 (MNV-1), a human norovirus (NoV) surrogate. The edible green and brown algae, fulvescens (Capsosiphon fulvescens) and fusiforme (Hizikia fusiforme), respectively, were experimentally contaminated with 5-6 log(10) plaque forming units (PFU)/ml MNV-1. The titer of MNV-1 significantly decreased (P < 0.05) as the dose of gamma radiation increased. MNV-1 titer decreased to 1.16-2.46 log(10) PFU/ml in fulvescens and 0.37-2.21 log(10) PFU/ml in fusiforme following irradiation. However, all Hunters ('L', 'a' and 'b') and sensory qualities (appearance, color, flavor, texture and overall acceptability) were not significantly (P > 0.05) different in both algae following gamma radiation. The Weibull model was used to generate non-linear survival curves and to calculate G(d) values for 1, 2, and 3 log(10) reductions of MNV-1 in fulvescens (R-2 = 0.992) and fusiforme (R-2 = 0.988). A G(d) value of 1 (90% reduction) corresponded to 2.89 and 3.93 kGy in fulvescens and fusiforme, respectively. A G(d) value of 2 (99% reduction) corresponded to 7.75 and 9.02 kGy in fulvescens and fusiforme, respectively, while a G(d) value of 3 (99.9% reduction) in fulvescens and fusiforme corresponded with 13.83 and 14.93 kGy of gamma radiation, respectively. A combination of gamma radiation at medium doses and other treatments could be used to inactivate >= 3 log(10) PFU/ml NoV in seaweed. The inactivation kinetics due to gamma radiation against NoV in these algae might provide basic information for use in seaweed processing and distribution. (C) 2015 Published by Elsevier Ltd.

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