Ultraviolet-C efficacy against a norovirus surrogate and hepatitis A virus on a stainless steel surface

초록

In this study, the effects of 10-300 mWs/cm(2) of ultraviolet radiation (UV-C) at 260 nm were investigated for the inactivation of two foodborne viruses: murine norovirus-1 (MNV-1; a human norovirus [NoV] surrogate) and hepatitis A virus (HAV). We used an experimentally contaminated stainless steel surface, a common food-contact surface, to examine the effects of low doses of UV-C radiation on MNV-1 and HAV titers. The modified Gompertz equation was used to generate non-linear survival curves and calculate d(R)-values as the UV-C dose of 90% reduction for MNV-1 (R-2 = 0.95, RMSE = 0.038) and HAV (R-2 = 0.97, RMSE = 0.016). Total MNV-1 and HAV titers significantly decreased (p < 0.05) with higher doses of UV-C. MNV-1 and HAV were reduced to 0.0-4.4 and 0.0-2.6 log(10)PFU/ml, respectively, on the stainless steel surfaces by low-dose UV-C treatment. The d(R)-value, 33.3 mWs/cm(2) for MNV-1 was significantly (p < 0.05) lower than 55.4 mWs/cm(2) of HAV. Therefore, the present study shows that HAV is more resistant to UV-C radiation than MNV-1. These data suggest that low doses of UV-C light on food contact surfaces could be effective to inactivate human NoV and HAV in restaurant, institutional, and industrial kitchens and facilities. (C) 2015 Elsevier B.V. All rights reserved.

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